MKMaize KernelSpatial Atlas

Study & data

A spatial view of maize kernel filling.

This resource accompanies a spatial transcriptomic analysis of maize kernels across grain filling, providing electronic RNA in situ visualisation for genome-wide gene expression.

How to cite

Fu, Y., Xiao, W., Tian, L. et al. Spatial transcriptomics uncover sucrose post-phloem transport during maize kernel development. Nature Communications 14, 7191 (2023). https://doi.org/10.1038/s41467-023-43006-7

Please cite the original article when using this atlas or its visualisations. The article is open access under CC BY 4.0.

Biological question

How is sucrose distributed after phloem unloading?

The maize kernel brings together maternal tissue, filial endosperm and embryo. During filling, the transfer of carbon through the maternal–filial interface supports the accumulation of starch and protein in endosperm and lipids in embryo.

By combining microscopic sectioning with spatial transcriptomics, the study identifies the spatial organisation of kernel cell populations and gene programs that support nutrient delivery and reserve formation.

Study workflow for spatial transcriptomics in maize kernels
Spatial transcriptomic workflow used in the study

Atlas outputs

Electronic RNA in situ maps

Gene expression is visualised in tissue context, enabling exploration of marker genes and spatial patterns without requiring experimental in situ hybridisation for every target.

Key finding

Post-phloem transport

Maternal placento-chalazal tissue and the basal endosperm transfer layer form specialised interfaces that support nutrient uptake and downstream storage programmes.

Resources

Data and related materials